Journal: Pathogens

Article Title: Effect of Probiotics on Host-Microbiota in Bacterial Infections

doi: 10.3390/pathogens11090986

Figure Lengend Snippet: Summary of clinical trials reporting the probiotics’ potential to counteract the impact and/or regulate the gut microbiota after infection.

Article Snippet: Randomized, double-blind, placebo-controlled study , N = 30 18–56 y/o , To analyze the effect of PPI-induced gastric acid suppression on the survival and colonization of a multi-strain probiotic mix (VSL Pharmaceuticals, Inc. USA, batch no: 710012) , Acid suppression enhances certain probiotic-associated bacterial colonization and probiotics in turn suppressed PPI-mediated intestinal microbial alterations. Increased microbial abundance of Streptococcaceae ( p = 0.004), Leuconostacaceae ( p = 0.001), and Pasteurellaceae ( p = 0.020) families , [ ] .

Techniques: Clinical Proteomics, Probiotics, Infection, Bacteria, Metabolic Labelling, Isolation, Activity Assay

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: An insight into the functional alterations in the gut microbiome of healthy adults in response to a multi-strain probiotic intake: a single arm open label trial

doi: 10.3389/fcimb.2023.1240267

Figure Lengend Snippet: The effects of multi-strain probiotic intervention on abundance distribution of family Odoribacteraceae and Bacteriodaceae and the genus Megamonas . Abundance of Odoribacteraceae (A) , Bacteriodaceae (B) and Megamonas (C) in fecal samples collected at baseline (Day 0) and at the end of multi-strain probiotic intervention (Day 56 [ ± 2 days]) were compared by taxonomic analysis. (*) p < 0.05.

Article Snippet: The multi-strain probiotic was supplied by ADM Protexin as capsules that were administered orally.

Techniques:

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: An insight into the functional alterations in the gut microbiome of healthy adults in response to a multi-strain probiotic intake: a single arm open label trial

doi: 10.3389/fcimb.2023.1240267

Figure Lengend Snippet: Heatmap and contingency table of detected multi-strain probiotic representatives. Left panel differential abundance species between baseline (Day 0) and end of multi-strain probiotic intervention on Day 56 ( ± 2 days). Species are shown over‐represented (red color) or under‐represented (blue color) in Day 56 respect to Day 0. Right panel, the number of participants in which the probiotic strains abundance was different from zero. Data was analyzed using the Wilcoxon signed-rank test and p-values of < 0.05 were considered significant.

Article Snippet: The multi-strain probiotic was supplied by ADM Protexin as capsules that were administered orally.

Techniques:

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: An insight into the functional alterations in the gut microbiome of healthy adults in response to a multi-strain probiotic intake: a single arm open label trial

doi: 10.3389/fcimb.2023.1240267

Figure Lengend Snippet: Functional alpha-diversity analysis expressed as values and as changes from baseline to the end of multi-strain probiotic intervention at species level. (A) Boxplots of four common alpha-diversity indices (Richness, Simpson, Shannon and Pielou’s evenness index) at baseline (Day 0) and end of multi-strain probiotic intervention on Day 56 ( ± 2 days). (B) Violin graph of delta alpha-diversity indexes (between Day 56 and Day 0 values) of the four alpha-diversity indices. Data was analyzed using the Wilcoxon signed-rank test and p-values of < 0.05 were considered significant. (C) Gene set enrichment analysis was performed to compare Kyoto Encyclopedia of Genes and Genomes pathways from fecal samples provided at baseline (Day 0) and end of multi-strain probiotic intervention on Day 56 ( ± 2 days). Pathways categories are shown over‐represented (red color) or underrepresented (blue color) in Day 56 respect to Day 0. (#) p > 0.1; (*): p < 0.05. L1: high-level functions and utilities; L2: General categories; L3: Pathway level. NES, Normalized Enrichment Scores.

Article Snippet: The multi-strain probiotic was supplied by ADM Protexin as capsules that were administered orally.

Techniques: Functional Assay

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: An insight into the functional alterations in the gut microbiome of healthy adults in response to a multi-strain probiotic intake: a single arm open label trial

doi: 10.3389/fcimb.2023.1240267

Figure Lengend Snippet: Comparison of hydrogen and methane production between baseline and end of multi-strain probiotic intervention. Participants were required to provide a fasted breath sample at baseline (Day 0) and during end of study visit (Day 56 ± 2 days). Hydrogen (A) and methane (B) values were determined from breath samples analyzed using gas chromatography to compare baseline (Day 0) and end of study (Day 56) readings. Data was analyzed using the Wilcoxon signed-rank test and p-values of <0.05 were considered significant. CH4, methane; H2, hydrogen.

Article Snippet: The multi-strain probiotic was supplied by ADM Protexin as capsules that were administered orally.

Techniques: Comparison, Gas Chromatography

Journal: Journal of Translational Medicine

Article Title: Nutraceuticals and pharmacological to balance the transitional microbiome to extend immunity during COVID-19 and other viral infections

doi: 10.1186/s12967-024-05587-9

Figure Lengend Snippet: Properties of Nutraceuticals and Pharmacological interventions to regulate the protective immune activities in alveolar and gut cell linings. A probable modulatory effect of probiotics with supplements to build ILC 3 homeostasis in gut and the functional integrity of the other body organs [ , , – , ]

Article Snippet: NCT04877704 , The effects of symprove, a multi-strain Probiotic, as an Adjuvant in the Management of COVID-19 in Hospitalized Patients , Symprove probiotics , Length of Hospital Stay, intestinal inflammation, symptoms recovery in 3 months.

Techniques: Probiotics, Functional Assay

Journal: Journal of Translational Medicine

Article Title: Nutraceuticals and pharmacological to balance the transitional microbiome to extend immunity during COVID-19 and other viral infections

doi: 10.1186/s12967-024-05587-9

Figure Lengend Snippet: The interventional studies are being carried out using probiotics in COVID-19 individuals with expected outcome (ClinicalTrials.gov—U.S. National Library of Medicine)

Article Snippet: NCT04877704 , The effects of symprove, a multi-strain Probiotic, as an Adjuvant in the Management of COVID-19 in Hospitalized Patients , Symprove probiotics , Length of Hospital Stay, intestinal inflammation, symptoms recovery in 3 months.

Techniques: Probiotics, Infection, Maltodextrin, Starch, Capsules, Bacteria, Activation Assay, Biomarker Assay, Adjuvant

Journal: International Journal of Medical Sciences

Article Title: Multi-Strain Probiotics Inhibit Cardiac Myopathies and Autophagy to Prevent Heart Injury in High-Fat Diet-Fed Rats

doi: 10.7150/ijms.14769

Figure Lengend Snippet: Masson's trichrome staining of cardiac tissue sections. Histopathological analysis of cardiac tissue sections of the left ventricle from the control rats, the high-fat diet-induced obese rats, the high-fat diet-fed rats treated with low dose, medium dose and high dose of multi-strain probiotics. White arrow: site of collagen accumulation.

Article Snippet: Multi-strain probiotic powder ( Lactobacillus rhamnosus: Pediococcus acidilactici : Bifidobacterium adolescentis ; 1: 1: 1) was produced by freeze-drying and obtained from New Bellus Enterprise Co., Ltd (Tainan, Taiwan).

Techniques: Staining, Control, Probiotics

Journal: International Journal of Medical Sciences

Article Title: Multi-Strain Probiotics Inhibit Cardiac Myopathies and Autophagy to Prevent Heart Injury in High-Fat Diet-Fed Rats

doi: 10.7150/ijms.14769

Figure Lengend Snippet: Fibrosis-related protein expression analysis via Western blot. The fibrosis-related protein levels were increased in the left ventricular tissue of high-calorie diet-induced obese rats, whereas treatment with different doses of multi-strain probiotics reduced these fibrosis-related proteins. (A) Western blots for the fibrosis pathway proteins. Quantitative analysis of the expression levels of (B) MMP2 or (C) TGF-β normalized to those of tubulin, #P<0.05, ##P<0.01 or ###P<0.001: the mean values were significantly different from those of the control group.

Article Snippet: Multi-strain probiotic powder ( Lactobacillus rhamnosus: Pediococcus acidilactici : Bifidobacterium adolescentis ; 1: 1: 1) was produced by freeze-drying and obtained from New Bellus Enterprise Co., Ltd (Tainan, Taiwan).

Techniques: Expressing, Western Blot, Probiotics, Control

Journal: International Journal of Medical Sciences

Article Title: Multi-Strain Probiotics Inhibit Cardiac Myopathies and Autophagy to Prevent Heart Injury in High-Fat Diet-Fed Rats

doi: 10.7150/ijms.14769

Figure Lengend Snippet: Autophagy early stage-related protein expression analysis via Western blot. The autophagy-related protein levels were increased in the left ventricular tissue of high-calorie diet-induced obese rats, whereas treatment with different doses of multi-strain probiotics reduced these fibrosis-related proteins. (A) Western blots for the fibrosis pathway proteins. Quantitative analysis of the expression levels of (B) LC3B or (C) ATG7 normalized to those of tubulin, #P<0.05, ##P<0.01 or ###P<0.001: the mean values were significantly different from those of the control group.

Article Snippet: Multi-strain probiotic powder ( Lactobacillus rhamnosus: Pediococcus acidilactici : Bifidobacterium adolescentis ; 1: 1: 1) was produced by freeze-drying and obtained from New Bellus Enterprise Co., Ltd (Tainan, Taiwan).

Techniques: Expressing, Western Blot, Probiotics, Control

Journal: International Journal of Medical Sciences

Article Title: Multi-Strain Probiotics Inhibit Cardiac Myopathies and Autophagy to Prevent Heart Injury in High-Fat Diet-Fed Rats

doi: 10.7150/ijms.14769

Figure Lengend Snippet: ICH staining of cardiac tissue sections. Histopathological analysis of cardiac tissue sections of the left ventricle from the control rats, the high-fat diet-induced obese rats, the high-fat diet-fed rats treated with low dose, medium dose and high dose of multi-strain probiotics. Brown color: site of LC3B expression.

Article Snippet: Multi-strain probiotic powder ( Lactobacillus rhamnosus: Pediococcus acidilactici : Bifidobacterium adolescentis ; 1: 1: 1) was produced by freeze-drying and obtained from New Bellus Enterprise Co., Ltd (Tainan, Taiwan).

Techniques: Staining, Control, Probiotics, Expressing

Journal: International Journal of Medical Sciences

Article Title: Multi-Strain Probiotics Inhibit Cardiac Myopathies and Autophagy to Prevent Heart Injury in High-Fat Diet-Fed Rats

doi: 10.7150/ijms.14769

Figure Lengend Snippet: Hypertrophy-related protein expression analysis via Western blot. The autophagy-related protein levels were increased in the left ventricular tissue of high-calorie diet-induced obese rats, whereas treatment with different doses of multi-strain probiotics reduced these fibrosis-related proteins. (A) Western blots for the fibrosis pathway proteins. Quantitative analysis of the expression levels of (B) ERK5, (C) MEK5, (D) uPA and (E) ANP normalized to those of tubulin, #P<0.05, ##P<0.01 or ###P<0.001: the mean values were significantly different from those of the control group.

Article Snippet: Multi-strain probiotic powder ( Lactobacillus rhamnosus: Pediococcus acidilactici : Bifidobacterium adolescentis ; 1: 1: 1) was produced by freeze-drying and obtained from New Bellus Enterprise Co., Ltd (Tainan, Taiwan).

Techniques: Expressing, Western Blot, Probiotics, Control

Journal: PLoS ONE

Article Title: Oral probiotic combination of Lactobacillus and Bifidobacterium alters the gastrointestinal microbiota during antibiotic treatment for Clostridium difficile infection

doi: 10.1371/journal.pone.0204253

Figure Lengend Snippet: Quantity of samples collected during the PICO study.

Article Snippet: After the enrollment period, 33 subjects experiencing a first episode of mild to moderate CDI were randomly assigned an oral placebo or multi-strain probiotic capsule ( Lactobacillus acidophilus NCFM, ATCC 700396; Lactobacillus paracasei Lpc-37, ATCC SD5275; Bifidobacterium lactis Bi-07, ATCC SC5220; Bifidobacterium lactis B1-04, ATCC SD5219) containing 1.7 x 10 10 CFU/capsule daily for four weeks (week 0 to week 4).

Techniques:

Journal: PLoS ONE

Article Title: Oral probiotic combination of Lactobacillus and Bifidobacterium alters the gastrointestinal microbiota during antibiotic treatment for Clostridium difficile infection

doi: 10.1371/journal.pone.0204253

Figure Lengend Snippet: The Shannon diversity index, which is a heterogeneity measure that combines richness and evenness components of microbial diversity does not significantly differ when subjects take probiotics.

Article Snippet: After the enrollment period, 33 subjects experiencing a first episode of mild to moderate CDI were randomly assigned an oral placebo or multi-strain probiotic capsule ( Lactobacillus acidophilus NCFM, ATCC 700396; Lactobacillus paracasei Lpc-37, ATCC SD5275; Bifidobacterium lactis Bi-07, ATCC SC5220; Bifidobacterium lactis B1-04, ATCC SD5219) containing 1.7 x 10 10 CFU/capsule daily for four weeks (week 0 to week 4).

Techniques: Probiotics

Journal: PLoS ONE

Article Title: Oral probiotic combination of Lactobacillus and Bifidobacterium alters the gastrointestinal microbiota during antibiotic treatment for Clostridium difficile infection

doi: 10.1371/journal.pone.0204253

Figure Lengend Snippet: (A) Subjects in the probiotic group had a significantly lower abundance of the bacterial family Verrucomicrobiaceae at week 8 than placebo treated subjects; (B) Members of the bacterial genus Bacteroides were significantly reduced in abundance between weeks 0 to 4 in probiotic treated subjects; (C) Ruminococcus (family Lachnospiraceae) tended to be more abundant at week 8 than week 4 within the placebo group and at week 8 than week 0 within the probiotic group; * 0.05 < p < 0.1 and ** p ≤ 0.05.

Article Snippet: After the enrollment period, 33 subjects experiencing a first episode of mild to moderate CDI were randomly assigned an oral placebo or multi-strain probiotic capsule ( Lactobacillus acidophilus NCFM, ATCC 700396; Lactobacillus paracasei Lpc-37, ATCC SD5275; Bifidobacterium lactis Bi-07, ATCC SC5220; Bifidobacterium lactis B1-04, ATCC SD5219) containing 1.7 x 10 10 CFU/capsule daily for four weeks (week 0 to week 4).

Techniques:

Journal: PLoS ONE

Article Title: Multiple strains probiotics appear to be the most effective probiotics in the prevention of necrotizing enterocolitis and mortality: An updated meta-analysis

doi: 10.1371/journal.pone.0171579

Figure Lengend Snippet: Characteristics of the trials included in the analysis.

Article Snippet: The mortality rate in the probiotics group with all the tested probotics, including multiple strains probiotics, Lactobacillus, Bifidobacterium, and Saccharomyces, was 5.3%, whereas in the placebo group it was 6.9%.

Techniques: Probiotics

Journal: PLoS ONE

Article Title: Multiple strains probiotics appear to be the most effective probiotics in the prevention of necrotizing enterocolitis and mortality: An updated meta-analysis

doi: 10.1371/journal.pone.0171579

Figure Lengend Snippet: Meta-analysis of the efficacy of probiotics associated with the risk of NEC in preterm infants.

Article Snippet: The mortality rate in the probiotics group with all the tested probotics, including multiple strains probiotics, Lactobacillus, Bifidobacterium, and Saccharomyces, was 5.3%, whereas in the placebo group it was 6.9%.

Techniques: Probiotics

Journal: PLoS ONE

Article Title: Multiple strains probiotics appear to be the most effective probiotics in the prevention of necrotizing enterocolitis and mortality: An updated meta-analysis

doi: 10.1371/journal.pone.0171579

Figure Lengend Snippet: Meta-analysis of the efficacy of probiotics associated with the risk of death in preterm infants.

Article Snippet: The mortality rate in the probiotics group with all the tested probotics, including multiple strains probiotics, Lactobacillus, Bifidobacterium, and Saccharomyces, was 5.3%, whereas in the placebo group it was 6.9%.

Techniques: Probiotics